A new extraction procedure of autonomous DNA from eucaryotic cells, where DNA could be bound to proteins
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چکیده
منابع مشابه
A new extraction procedure of autonomous DNA from eucaryotic cells, where DNA could be bound to proteins.
The classical method for extraction of episomes from eucaryotic cells has been described by B.Hirt (1). It is based on precipitation of proteins associated to cellular DNA by sodium chloride, whereas the episomal DNA remains in the supernatant. However, this method does not allow to purify an eventual fraction of autonomous DNA which would be strongly associated with a protein as it is the case...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.18.5571